Skip Navigation



Annals of Oncology Advance Access published online on November 15, 2005
Annals of Oncology, doi:10.1093/annonc/mdj064
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
17/1/110    most recent
mdj064v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Karube, K.
Right arrow Articles by Harada, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karube, K.
Right arrow Articles by Harada, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2005 European Society for Medical Oncology
Received April 12, 2005
Revised September 29, 2005
Accepted October 3, 2005

original article

Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed-Sternberg (HRS) cells: high expression of interleukin-11 receptor {alpha}

K. Karube 1 *, K. Ohshima 2, J. Suzumiya 3, R. Kawano 2, M. Kikuchi 2, and M. Harada 4

1 Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan; Department of Medicine and Biosystemic Science, Internal Medicine, Medicine and Surgery, Kyushu University Graduate School of Medical Science, Fukuoka, Japan
2 Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan
3 Department of Internal Medicine, School of Medicine, Fukuoka University, Fukuoka, Japan
4 Department of Medicine and Biosystemic Science, Internal Medicine, Medicine and Surgery, Kyushu University Graduate School of Medical Science, Fukuoka, Japan

* To whom correspondence should be addressed.
K. Karube, E-mail: karube1975{at}yahoo.co.jp


  Abstract

We microdissected Hodgkin and Reed-Sternberg (HRS) cells from 14 Hodgkin's lymphoma tissue samples (nodular sclerosis = 5; mixed cellularity = 9), and after isolation and amplification of mRNA, analyzed the expression profile of 140 genes of chemokines, cytokines and their receptors by cDNA microarray methods. We also compared the profile with those of germinal center (GC) cells in reactive lymphadenitis. Unsupervised clustering revealed a relatively homogeneous expression profile in HRS cells. HRS cells tended to express mainly Th2 T cell-associated molecules rather than those of Th1, compared with GC cells. Interleukin-11 receptor {alpha} (IL-11R{alpha}), a previously unknown HRS cell-specific gene, was detected in addition to known genes. Immunohistochemical staining confirmed the expression of IL-11R{alpha} at the protein level. In contrast, only few cases were positive for IL-11R{alpha} in B cell lymphoma, diffuse large cell lymphoma and follicular lymphoma. This is the first analysis report of tissue HRS cells with cDNA microarray technique.

Keywords: Hodgkin; Reed-Sternberg; chemokine; microdissection; cDNA microarray.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
F. A. Scheeren, S. A. Diehl, L. A. Smit, T. Beaumont, M. Naspetti, R. J. Bende, B. Blom, K. Karube, K. Ohshima, C. J. M. van Noesel, et al.
IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis
Blood, May 1, 2008; 111(9): 4706 - 4715.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.