© 2007 European Society for Medical Oncology
gynecologic tumors |
Frequency and prognostic significance of HPV DNA in sentinel lymph nodes of patients with cervical cancer
1 Departments of Histology and Tumor Biology
2 Gynecology and Obstetrics
3 Pathology, Hôpital Tenon, Assistance Publique des Hôpitaux de Paris, CancerEst, Université Pierre et Marie Curie Paris VI, Paris, France
* Correspondence to: Prof. E. Daraï, Department of Gynecologic and Obstetrics, Hôpital Tenon, 4 rue de la Chine, 75020 Paris, France. Tel: +33-156-016-849; Fax: +33-156-016-062; E-mail: emile.darai{at}tnn.aphp.fr
Background: It has been suggested that histologically undetectable or ‘occult’ metastases in the lymphatic system could explain some recurrences. HPV DNA screening by means of the polymerase chain reaction (PCR) has been proposed as a method to detect occult metastases. This study was designed to determine the frequency of HPV DNA detection by PCR in sentinel lymph node (SN), and its relation to the clinical characteristics and outcome of women with cervical cancer.
Patients and methods: The primary cervical tumor and SN were tested for HPV DNA by means of PCR in 59 patients.
Results: Fifteen (25.4%) of the 59 women undergoing the SN procedure had an involved SN. HPV DNA was more frequent in positive SN than in negative SN (P < 0.0001). Seven patients had a recurrence, after a mean delay of 17 months (range: 10–26). One of seven patients with a recurrence had an involved SN. HPV DNA was detected in an SN of one of seven patients with recurrence and nine (19.5%) of 46 patients without recurrence (not significant).
Conclusion: In women with cervical cancer, HPV DNA screening of sentinel nodes might help to identify patients at risk of lymph node metastases and recurrence.
Key words: cervical cancer, sentinel lymph node, human papillomavirus, polymerase chain reaction
Received for publication February 9, 2007. Revision received March 26, 2007. Revision received April 6, 2007. Accepted for publication April 11, 2007.