Annals of Oncology Advance Access originally published online on March 12, 2007
Annals of Oncology 2007 18(6):1043-1050; doi:10.1093/annonc/mdm072
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© 2007 European Society for Medical Oncology
lung cancer |
Immunohistochemichal expression of biomarkers: a comparative study between diagnostic bronchial biopsies and surgical specimens of non-small-cell lung cancer
1 Department of Medicine, Institut Gustave Roussy (IGR), Villejuif
2 Institut National de la Santé et de la Recherche Médicale UMR 484 and Department of Pathology, Centre Jean Perrin, Clermont-Ferrand
3 Department of Biostatistics
4 Laboratory of Translational Research, BU Thoracic/Head and Neck Cancer, IGR, Villejuif
5 Department of Pathology
6 Department of Pneumology, Institut Mutualiste Montsouris
7 Department of Thoracic Surgery, Hôpital Tenon, Paris, France
* Correspondence to: Dr J.-C. Soria, Department of Cancer Medicine, Institut Gustave Roussy, 39 Rue Camille Desmoulins, 94805 Villejuif, France. Tel: +33-4211-4291; Fax: +33-4211-5230; E-mail: soria{at}igr.fr
Background: The increasing use of biomarkers as molecular determinants of responsiveness to conventional chemotherapy or molecular targeted therapy has raised the question of the reliability and reproducibility of their evaluation in bronchial biopsies as compared with corresponding resected surgical specimens.
Patients and methods: Immunohistochemical expression of five markers related to signal transduction [epidermal growth factor receptor (EGFR), phospho-Akt], cell proliferation (Ki-67), DNA repair [excision repair cross-complementing (ERCC)1] and cellular ‘immortality’ [human telomerase catalytic component (hTERT)], was assessed in 41 patients with operable non-small-cell lung cancer in both bronchial biopsies and whole surgical specimens.
Results: High correlation coefficients were observed between the expression of ERCC1, hTERT and Ki-67 in the biopsies and the surgical specimens [0.83 (P < 0.0001); 0.55 (P < 0.001) and 0.64 (P < 0.0001), respectively]. On the other hand, biomarker expression in biopsy was less correlated with the expression in the whole tissue sample for the markers of signal response and transduction [0.24 (P = 0.17) and 0.29 (P = 0.09) for EGFR and phospho-Akt, respectively].
Conclusions: Our results indicate a lack of association in the expression of important biomarkers between lung biopsies and corresponding resected tumors, with discordance rates ranging between 9% and 41%. Although these results need to be further validated in larger cohorts, they indicate that the evaluation of the expression of biomarkers in bronchial biopsies can be misleading.
Key words: biological markers, biopsy, correlation, immunohistochemistry, non-small-cell lung cancer, surgical specimen
Received for publication October 11, 2006. Revision received January 30, 2007. Accepted for publication February 1, 2007.
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