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Annals of Oncology Advance Access originally published online on September 9, 2007
Annals of Oncology 2007 18(11):1804-1809; doi:10.1093/annonc/mdm356
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© 2007 European Society for Medical Oncology

gynecologic tumors

HLA-G expression in human ovarian carcinoma counteracts NK cell function

A. Lin1,2, W.-H. Yan1,*, H.-H. Xu1, M.-F. Gan3, J.-F. Cai3, M. Zhu1 and M.-Y. Zhou1

1 Medical Research Center
2 Human Tissue Bank
3 Department of Pathology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, People's Republic of China

* Correspondence to: Dr W.-H. Yan, Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang, 317000, People's Republic of China. Tel: +86–576–85199347; E-mail: yanwhcom{at}yahoo.com

Background: Human leukocyte antigen-G (HLA-G) is an important immunotolerant which could be a part of the strategies applied by malignant cells applied to avoid host immunosurveillance. Aberrant expression of HLA-G has been found in ovarian carcinoma. The aim of this study was to evaluate the HLA-G expression in ovarian cancer tissues and to explore its function in vitro.

Materials and methods: HLA-G expression in 33 primary ovarian carcinoma tissues was analyzed using immunohistochemistry with the anti-HLA-G monoclonal antibody (mAb) 4H84. Furthermore, the function of HLA-G in NK cell cytotoxicity was determined in vitro by cloning and expression of HLA-G on the ovarian carcinoma cell OVCAR-3.

Results: HLA-G expression was detected in 22/33 (66.7%) primary tumor tissues, but was absent in normal ovarian tissues (P<0.01). Cytotoxicity studies showed that HLA-G expression dramatically inhibits cell lyses by NK-92 cells (P<0.01), which could be restored by the anti-HLA-G conformational mAb 87G (P<0.01).

Conclusion: HLA-G was expressed in a significant number of primary ovarian carcinoma tissues, and HLA-G expression in OVCAR-3 could directly inhibit NK-92 cell lysis. Taken together, our results indicated that expression of HLA-G plays an important role in evasion of ovarian cancer cells from host immunosurveillance.

Key words: HLA-G, immune response, NK cell, ovarian carcinoma

Received for publication April 27, 2007. Accepted for publication June 11, 2007.


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