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Annals of Oncology Advance Access originally published online on November 15, 2005
Annals of Oncology 2006 17(1):110-116; doi:10.1093/annonc/mdj064
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© 2005 European Society for Medical Oncology

Gene expression profile of cytokines and chemokines in microdissected primary Hodgkin and Reed–Sternberg (HRS) cells: high expression of interleukin-11 receptor {alpha}

K. Karube1,2,*, K. Ohshima1, J. Suzumiya3, R. Kawano1, M. Kikuchi1 and M. Harada2

Departments of 1 Pathology and 3 Internal Medicine, School of Medicine, Fukuoka University, Fukuoka; 2 Department of Medicine and Biosystemic Science, Internal Medicine, Medicine and Surgery, Kyushu University Graduate School of Medical Science, Fukuoka, Japan

* Correspondence to: Dr K. Karube, Department of Pathology, School of Medicine, Fukuoka University, Nanakuma 7-45-1, Jonan-ku, Fukuoka 814-0180, Japan. Tel: +81-942-31-7547; Fax: +81-92-861-7300; E-mail: karube1975{at}yahoo.co.jp

We microdissected Hodgkin and Reed–Sternberg (HRS) cells from 14 Hodgkin's lymphoma tissue samples (nodular sclerosis = 5; mixed cellularity = 9), and after isolation and amplification of mRNA, analyzed the expression profile of 140 genes of chemokines, cytokines and their receptors by cDNA microarray methods. We also compared the profile with those of germinal center (GC) cells in reactive lymphadenitis. Unsupervised clustering revealed a relatively homogeneous expression profile in HRS cells. HRS cells tended to express mainly Th2 T cell-associated molecules rather than those of Th1, compared with GC cells. Interleukin-11 receptor {alpha} (IL-11R{alpha}), a previously unknown HRS cell-specific gene, was detected in addition to known genes. Immunohistochemical staining confirmed the expression of IL-11R{alpha} at the protein level. In contrast, only few cases were positive for IL-11R{alpha} in B cell lymphoma, diffuse large cell lymphoma and follicular lymphoma. This is the first analysis report of tissue HRS cells with cDNA microarray technique.

Key words: Hodgkin, Reed–Sternberg, chemokine, microdissection, cDNA microarray


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