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Annals of Oncology 8:S55-S57, 1997
© 1997 European Society for Medical Oncology

Detection of trisomy 12 in CD34+ progenitor cells in a patient with B-cell chronic lymphocytic leukemia by florescence in situ hybridization

B. Gahn, C. Schäfer, J. Neef, C. Troff, M. Feuring-Buske, W. Hiddemann and B. Wörmann

Department of Haematology and Oncology, University of Göttingen Göttingen, Germany

Correspondence to: Dr. Benedikt Gahn Department of Hematology and Oncology University of Göttingen Robert-Koch-Straβe 40 D-37 075 Göttingen, Germany

B-cell chronic lymphocytic leukemia (B-CLL) is a slowly progressive disease resulting from the clonal expansion of mature B lymphocytes. The most frequent chromosomal abnormality is trisomy 12. Recently more aggressive therapeutic approaches using myeloablative therapy and autologous stem-cell support have been developed. Phase I/II studies have resulted in molecular remission and prolonged survival. One cause of relapse may be tumor-cell contamination of the transplant. We asked whether immunophenotypically identified hematopoietic progenitor cells are part of the malignant cell population in B-CLL. In a patient with trisomy 12, two subpopulations of hematopoietic progenitor cells, CD34+/CD38+ and CD34+/CD38– cells, were isolated by fluorescence-activated-cell-sorting; the sort purity was 98%. Trisomy 12 was detected in 13% of CD34+/38– cells and in 34% of CD34+/38– cells. These data suggest that CD34+ cells are involved in the malignant clone of patients with B-CLL. The results are of significance for future strategies using autologous stem-cell transfusion.

CD34+ progenitor cells, chronic lymphocytic leukemia, trisomy 12


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