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Annals of Oncology 8:S33-S36, 1997
© 1997 European Society for Medical Oncology

Specific detection of Helicobactor pylori and non-Helicobactor pylori flora in small-and large-cell primary gastric B-cell non-Hodgkin's lymphoma

I. A. M. Gisbertz1,4, D. M. A. E. Jonkers2,3, J. W. Arends4, F. J. Bot4, R. W. Stockbrügger2, L. W. Vrints5 and H. C. Schouten1

1 Department of Internal Medicine, Hematology/Oncology, University Hospital Maastricht the Netherlands
2 Department of Internal Medicine, Gastroenterology Sections, University Hospital Maastricht the Netherlands
3 Department of Internal Medicine, Microbiology, University Hospital Maastricht the Netherlands
4 Department of Internal Medicine, Pathology, University Hospital Maastricht the Netherlands
5 Department of Pathology, Catharina Hospital Eindhoven the Netherlands

Correspondence to: Harry C. Schouten, MD, PhD Department of Internal Medicine University Hospital Maastricht P.O. Box 5800 6202 AZ Maastricht The Netherlands

Background Primary gastric non-Hodgkin's lymphomas possibly develop in response to local infection by Helicobacter pylori (H. pylori). We investigated the presence of H. pylori and non-H. pylori flora histologically in small- and large-cell primary gastric lymphoma using a specific staining method.

Materials and methods Specimens of 52 cases of primary gastric lymphoma (17 small cell, 35 large cell) were stained with modified Giemsa (MG) and immunohistochemically using a polyclonal antibody against H. pylori (IHC).

Results Thirty-two cases (61.5%) (small cell 76% versus large cell 53%, P > 0.05) showed immunoreactivity for H. pylori in the mucosa surrounding the tumor. Remarkably, there was localization of H. pylori in the neck of the gastric glands in 3 cases. Non-H. pylori flora was seen in 35 cases (76.3%) (small cell 53% versus large cell 74%, P > 0.05). In 20 cases, this non-H. pylori flora was mixed with H. pylori. Five cases showed no bacterial flora at all.

Conclusions (l)Using immunohistochemistry, the prevalence of gastric lymphoma cases with H. pylori (61.5%) approximates that of H. pylori in the normal population. (2) No statistical difference was found between the occurrence of H. pylori and non-H. pylori bacterial flora in small- versus large-cell lymphoma. (3) Our results suggest that H. pylori may not be the only etiologic factor in primary gastric lymphoma.

bactorial flora, Helicobactor pylori, immunohistochemistry, MALT, primary gastric lymphoma


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