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Annals of Oncology 12:S27-S29, 2001
© 2001 European Society for Medical Oncology

Reviews

Expression of neuropeptide Y receptors in human prostate cancer cells

P. Magni and M. Motta

Center for Endocrinological Oncology Institute of Endocrinology University of Milan Milan, Italy

Correspondence to: P. Magni, MD PhD Istituto di Endocrinologia via G. Balzaretti, 9 20133 Milano, Italy E-mail: paolo.magni{at}unimi.it

Background: Neuroendocrine molecules are now believed to play a significant role in the progression of human prostate cancer (CaP), especially in the androgen-independent stage.

Materials and methods: In the present study, we evaluated the presence and the function of the receptors for neuropeptide Y (NPY) in human CaP cell lines (the androgen-dependent LNCaP, and the androgen-independent DU 145 and PC-3).

Results: The presence of high-affinity binding sites for NPY was shown on PC-3 cells (radioreceptor assay). Reverse transcription-polymerase chain reaction analysis indicated that these sites correspond to the Y1 and Y2 receptor isoforms. A Y1 receptor protein (70 kDa) was also detected in PC-3 cell extracts by Western blot analysis. The activation of these receptors by NPY resulted in a reduction of forskolin-induced cAMP accumulation and an increase of [Ca2+]i Moreover, a prolonged treatment with NPY induced a dose-related proliferation of PC-3 cells.

Conclusions By showing that NPY receptors are expressed in the androgen-independent cell line PC-3 and that their activation results in cell proliferation, the present date suggest that NPY-related mechanisms might be relevant in certain stages of CaP, such as the progression of the disease during the androgen-independent stage.

neuroendocrinology, neuropeptide Y, neuropeptide Y receptors, prostate cancer


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